Fig 2. Evaluation of PD-1 expression in lymphocytes from healthy and diseased dogs after stimulation with recombinant canine proteins.
PBMCs from healthy negative control dogs (n = 5) (A) and dogs with leishmaniasis (n = 10) (B) were cultured in medium alone (Medium) or medium containing rcaIL-12/rcaIL-2, rcaIL-12/rcaIL-15, rcaIL-12/rcasIL-10R1, rcaIL-15/rcaIL-7, or rcasIL-10R1 alone, with or without the addition of SLA. After 5 days, PMBCs were labeled with anti-human CD279 (PD-1) PE-conjugated monoclonal antibodies or a PE-conjugated isotype control. Lymphocyte mean fluorescence intensity (MFI) was assessed by flow cytometry. Bars represent MFI median values and 25th and 75th percentile interquartile range. Symbols represent data from individual animals. Asterisks indicate significant differences (Friedman’s test with Dunn’s multiple comparison, p < 0.05).