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. 2020 Jan 21;14(1):e0008021. doi: 10.1371/journal.pntd.0008021

Fig 5. Evaluation of TNF-α production in PBMCs from healthy and diseased dogs after stimulation with recombinant canine proteins.

Fig 5

PBMCs from healthy negative control dogs (n = 5) (A) and dogs with leishmaniasis (n = 10) (B) were cultured in medium alone (Medium) or medium containing rcaIL-12/rcaIL-2, rcaIL-12/rcaIL-15, rcaIL-12/rcasIL-10R1, rcaIL-15/rcaIL-7, or rcasIL-10R1 alone. These PBMCs were cultured with combinations of recombinant canine proteins with or without adding SLA. After 5 days, TNF-α concentration was determined in culture supernatants by capture ELISA. Bars represent cytokine concentration medians and 25th and 75th percentile interquartile range. Symbols represent data from individual animals. Asterisks indicate significant differences (Friedman’s test with Dunn’s multiple comparison, p < 0.05).