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(A) Wavenumber position of the benzene ring breathing mode of MBA-NP at different pH levels for nonburied particles using TRS. (B) Score intensities of principal component one versus pH level. (C) Loadings of principal component (PC) 1 of MBA-NP solution alone (Model) and the loadings of PC1 of MBA-NP when buried in porcine tissue (tissue thickness within optical path = 22 mm). (D) Prediction of pH level using leave one out cross validation from the scores intensities.
