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(A) Porcine tissue with an off-center hole for the quartz vial, thereby providing multiple depths (5, 8, and 12 mm) of signal retrieval depending on orientation with the laser beam for SORS measurements. (B) Raman spectra of MBA, as measured in the SORS modality, at three different depths, “1”, “2”, and “3” (5, 8, and 12 mm, respectively), when buried in porcine tissue. (C) Predicted pH levels using SORS vs actual pH levels using the PCA scores model.
