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Kd(F) for binary interactions involving autoinhibition. (A and B) Test case 4: ssDNA binding to an autoinhibited region in a dsDNA hairpin. (C and D) Test case 5: vinculin D1 domain (red) binding to a VBS (blue) buried within a talin rod domain. Panels B and D show the ratio of force-dependent Kd(F) to Kd,o0 for the ssDNA binding to the dsDNA hairpin (eq 1) and Vd1 binding to a VBS-containing talin domain (eq 2), respectively.
