Table 5. Tabulated Kinetic Parameters Associated with T1 to T2 Cu Electron Transfer in the RpNiR-Core and Y323F RpNiR-Corea.
RpNiR construct | klim (s–1) | Ks (mM) | Ki (mM) | klim/Ks (mM–1 s–1) |
---|---|---|---|---|
RpNiR-core | 3.5 ± 0.4 | 1.2 ± 0.3 | 1.8 × 101 ± 0.6 × 101 | 2.8 ± 0.8 |
Y323F RpNiR-core | 2.7 ± 0.4 | 0.8 ± 0.4 | 4.2 × 101 ± 2.9 × 101 | 3.4 ± 1.6 |
Data are shown ± error of fit.