Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (R. Norvegicus) | F344/NHsd (Male) | Envigo and University of Maryland | RRID:RGD_61109 | |
| Strain, strain background (R. Norvegicus) | HSD:HIV-1 (F344) (Male) | Envigo and University of Maryland | ||
| Cell line (H. sapiens) | HEK293T | ATCC | RRID:CVCL_0063 | |
| Transfected construct (R. Norvegicus) | Rac1 shRNA | Origene Technologies | Cat # TL712781 | |
| Recombinant DNA reagent | pCMVR8.74 | AddGene | RRID:Addgene_22036 | |
| Recombinant DNA reagent | pMD2.G | AddGene | RRID:Addgene_12259 | |
| Antibody | Anti-pPAK1 rabbit polyclonal (Thr423) | Cell Signaling Technology | RRID:AB_330220 | 1:1000 |
| Antibody | Anti-PAK1 rabbit polyclonal | Cell Signaling Technology | RRID:AB_330222 | 1:1000 |
| Antibody | Anti-pLIMK1 rabbit polyclonal (Thr507/508) | EMD Millipore | RRID:AB_568901 | 1:1000 |
| Antibody | Anti-LIMK1 rabbit polyclonal | Cell Signaling Technology | RRID:AB_2281332 | 1:1000 |
| Antibody | Anti-pCofilin rabbit polyclonal (Ser3) | Cell Signaling Technology | RRID:AB_330238 | 1:1000 |
| Antibody | Anti-Cofilin rabbit monoclonal | Cell Signaling Technology | RRID:AB_10622000 | 1:1000 |
| Antibody | Anti-pSSH1L rabbit polyclonal (Ser978) | ECM Biosciences | RRID:AB_10553849 | 1:500 |
| Antibody | Anti-SSH1 rabbit monoclonal | Cell Signaling Technology | RRID:AB_2798263 | 1:1000 |
| Antibody | Anti-Rac1 | Cell Biolabs | Included in #STA-401–1 | 1:6000 |
| Antibody | Anti-β-actin rabbit polyclonal | Sigma-Aldrich | RRID:AB_476693 | 1:6000 |
| Antibody | Anti-MAP2 rabbit polyclonal |
EMD Millipore | RRID:AB_91939 | 1:1000 |
| Antibody | Anti-pPAK1 rabbit polyclonal (Thr423) | Invitrogen | RRID:AB_2554427 | 1:25 |
| Antibody | Anti-NeuN rabbit monoclonal | Cell Signaling Technology | RRID:AB_2651140 | 1:400 |
| Antibody | Goat polyclonal anti-rabbit Alexa Fluor 568 | Invitrogen | RRID:AB_143157 | 1:250 |
| Peptide, recombinant protein | Rat CXCL12 | Peprotech | Cat# 400-32A | |
| Commercial assay or kit | Rac1 Activity Assay Kit | Cell Biolabs | Cat# STA-401–1 | |
| Chemical compound, drug | NSC23766 | Tocris | Cat# 2161 | |
| Software, algorithm | GraphPad Prism | GraphPad Software | RRID:SCR_015382 | Version 8.0 |
| Software, algorithm | Neurolucida 360 | MBF Biosciences | RRID:SCR_016788 | Version 2017.01.4 |
| Software, algorithm | Nuance Multispectral Imaging Systems | Nuance | RRID:SCR_015382 | Version 2.10 |
| Software, algorithm | Med-PC | Med Associates | RRID:SCR_012156 | Version 4.1 |
| Other | Helios Gene Gun | Bio-Rad | Cat# 1652411 | |
| Other | Phalloidin Alexa Fluor 488 | Invitrogen | Cat # A12379 | 1:400 |
| Other | Alexa Fluor conjugated tyramide 488 | Invitrogen | Cat # B40953 | |
| Other | Alexa Fluor conjugated tyramide 555 | Invitrogen | Cat# B40955 | |
| Other | DiI Stain | Invitrogen | Cat# D282 | |
| Other | Hoechst | Invitrogen | H3570 | 1 μg/mL |