Figure 6. SMPD3 overexpression did not alter the response of oral dysplasia and cancer cell lines to serum starvation or radiation but improved resistance to the EGFR inhibitor erlotinib.

For serum starvation, cells were counted after growing for 48 hours in 10% FBS-containing or serum-free media. The effect of SMPD3 overexpression on radiation and erlotinib response was assessed using clonogenic assays. Bars represent the mean and standard deviation. Statistical significance was determined using a two-way ANOVA with post-hoc Šidák correction. ^**** P < 0.0001. ns: not significant.