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. Author manuscript; available in PMC: 2020 Feb 7.
Published in final edited form as: Gastroenterology. 2017 Nov 21;154(4):1080–1095. doi: 10.1053/j.gastro.2017.11.002

Figure 5.

Figure 5

IGF1 is dysregulated in Jag1Ndr/Ndr and Alagille liver. (A) PCA of RNA sequencing reveals that Jag1+/+ and Jag1Ndr/Ndr liver transcriptomes cluster distinctly. (B) Heatmap shows 679 significantly up-regulated and 374 down-regulated genes in Jag1Ndr/Ndr livers. (C) Comparison of Gene Set Enrichment Analyses (GSEA), of livers from Jag1Ndr/Ndr mice and patients with ALGS (Supplementary Tables 12–15) shows extensive overlap. (D) Comparison of significantly dysregulated genes shows (D) 16 genes up-regulated and (E) 2 genes down-regulated in both Jag1Ndr/Ndr mice and ALGS, including Igf1. Igf1 mRNA levels are highly down-regulated in Alagille livers (F) and Jag1Ndr/Ndr livers (G). IGF1 protein levels were confirmed to be down-regulated in serum of (H) P10 and (I) adult mice. Error bars indicate s.d.; **P <.01, ***P <.001. (In F, and G, P-values are corrected P-values).