Table 1:
Effects of cystine influx upon transcripts of interest.
| Gene | Fold Induction | FDR | (regulator) |
|---|---|---|---|
| yhaO | 130 | 0 | Cysteine importer, desulfidase (YbaO control). |
| yhaM | 56 | 0 | Cysteine importer, desulfidase (YbaO control). |
| alaE | 44 | 6.6E-154 | Alanine exporter (Lrp). |
| dadA | 17 | 0 | D-amino acid dehydrogenase/alanine racemase (Lrp). |
| dadX | 16 | 0 | D-amino acid dehydrogenase/alanine racemase (Lrp). |
| ilvG | 6.5 | 1.4E-137 | Isoleucine biosynthetic operon (attenuation). |
| ilvM | 10 | 2.0E-106 | Isoleucine biosynthetic operon (attenuation). |
| ilvE | 3.2 | 3.6E-103 | Isoleucine biosynthetic operon (attenuation). |
| ilvD | 4.6 | 2.6E-78 | Isoleucine biosynthetic operon (attenuation). |
| ilvA | 4.7 | 1.3E-148 | Isoleucine biosynthetic operon (attenuation). |
| aaeA | 1.5 | 0.00041 | Efflux pump (Crp). |
| eamA | 1.3 | 4.5E-05 | Putative cysteine efflux pump. |
| eamB | 1.2 | 0.076 | Putative cysteine efflux pump. |
| yjcB | 5.9 | 6.8E-166 | Uncharacterized protein. |
| mdtK | 5.3 | 7.4E-121 | Multidrug efflux pump. |
| mdtM | 5.3 | 5.2E-80 | Multidrug efflux pump (SlyA). |
| yohC | 6 | 2.8E-266 | Inner-membrane protein. |
| tnaC | −11 | 5.1E-29 | leader peptide, tryptophanase |
| tnaA | −9 | 1.5E-113 | leader peptide, tryptophanase |
RNA sequencing analysis was performed upon triplicate samples of log-phase cells, with and without 10 min exposure to 50 μM cystine (Experimental Procedures). Fold induction denotes the level of gene mRNA shortly after cystine addition versus without cystine. Key transcriptional regulatory controls are indicated in parentheses; shaded genes belong to a common operon. See supplementary Table S1 for impact upon the CysB, MetJ, SOS, and OxyR regulons, and Table S2 for the full data set.