Tg expression assessed by FCM. (A-D) X-axis demonstrates hCD55 and
Y–axis demonstrates hCD47. (E, F) X-axis demonstrates HLA-E and Y-axis
demonstrates hCD46. A: PBMC of GalTKO. B: PBMC of
hCD55 Tg Sachs GalTKO pig, C: PBMC of hCD47/hCD55 Tg pig,
D: BM of hCD47/hCD55 Tg Sachs GalTKO pig. In hCD47/hCD55 Tg pig
PBMC and BM, both Tg were well expressed, whereas hCD47 expression was absent in
Sachs GalTKO and hCD55 GalTKO pigs. E: PBMC of GalTKO pig,
F: PBMC of hCD46/HLA-E Tg Revivicor pig, G: BM of
hCD46/HLA-E Tg Revivicor pig. The expression of HLA-E was weak in BM cells. BM:
bone marrow, FCM: flow cytometry, h: human, HLA: human leukocyte antigen, PBMC:
peripheral blood mononuclear cell, Tg: transgene.