Figure 5:

A: Number of spots of IFN gamma ELISPOT assays pre BM, at time of delayed lung Tx, and at the time of sacrifice from IBBMTx recipients. (1) 15P17, pre BM, 40 days following IBBMTx, at the time of sacrifice. (2) 14P3, pre BM, 70 days and at time of sacrifice, (3) 14P84, pre BM and 40 days, (4) 14P54 pre BM and 85 days, (5) 2715, pre BM, 28 days and at the time of sacrifice, (6) 14P55, pre BM and 60 days and at the time of sacrifice, and (7) 17214, pre BM, 25 days. Anti-BM and lung donor pig unresponsiveness was observed while anti-human response was uniformly preserved in all recipients prior to lung Tx in comparison with the results of pre BM without any conditioning. At the time of sacrifice, anti-lung donor unresponsiveness was observed in 15P17, 14P3,2715 and 14P55. Assays of baboons 14P3, 14P54 and 14P55 that were set up beyond 60 days still showed donor pig-specific unresponsiveness. Small responses against the third-party pig stimulator were seen. B: Stimulation indexes of MLR assays, pre BM and at the time of delayed lung Tx (4 baboons recipients of IBBMTx). (1) #15P17, pre BM and 40 days following IBBMTx, (2) 2715 pre BM and 28 days, (3) 14P55 pre BM and 60 days and (4)17214 pre BM and 25 days. Although 15P17 had general unresponsiveness, the other three demonstrated anti-human responses at the pre lung period. 14P55 and 17214 had anti-pig-specific unresponsiveness while 2715 showed donor pig-specific unresponsiveness in comparison with the results of pre BM.

Target cells: medium (M), self (S), bone marrow donor (BM), lung donor (LD), third party pig (3P) and human (H). NT: not tested.