Skip to main content
. Author manuscript; available in PMC: 2020 Jul 13.
Published in final edited form as: Nat Neurosci. 2020 Jan 13;23(2):209–216. doi: 10.1038/s41593-019-0567-0

Extended Data Fig. 1: Optogenetic inhibition of spontaneous activity in the VTA, in the absence of behavior.

Extended Data Fig. 1:

(A) (Top) Illustration of recording with a 64 electrode silicon microprobe during optogenetic inhibition of VTA DA neurons. (Bottom) Silicon microprobe with attached optical fiber under bright field and laser illumination. Scale bars: 0.2 mm.

(B) Spike raster (top) and mean firing rate versus time (bottom) of two VTA neurons in response to optogenetic inhibition. Orange bar indicates the duration of the laser stimulus. Insets show the spike waveform of each unit in blue (scale bar: 1 ms). Note the rebound excitation effect by the left unit.

(C) Firing rate modulation index distribution of 142 VTA neurons from 4 mice in response to optogenetic inhibition. The mean value was significantly less than zero (two-sided paired t-test, t141 = −5.6, P < 0.0001). Data are expressed as mean ± SEM.