Figure 4: MART1 TCR^bright T cells are reduced in the spleens of mice transplanted with A2⁻ or swine compared to A2⁺ thymus. a-d.

Quantification of spleen chimerism, analyzed 10–14 weeks post-transplant, including a. proportions of huCD45⁺ cells among total CD45⁺, b. percentages of GFP⁺ cells within huCD45⁺ cells, and c. proportions of CD3⁺ and d. CD4⁺CD3⁺ and CD8⁺CD3⁺ splenocytes (SPCs), respectively. * = p < 0.05, ** = p < 0.01 by the Kruskal-Wallis test using Dunn’s correction for multiple comparisons. Representative FCM staining of spleens of mice transplanted with MART1 TCR-transduced cells and cotransplanted with either e. human HLA-A2⁺, f. swine or g. human HLA-A2⁻ thymus, showing MART1 tetramer staining of huCD45⁺CD3⁺ CD4⁺ or CD8⁺GFP⁺ gated cells. Percent of MART1 TCR^bright cells among h. CD4⁺GFP⁺ and CD8⁺GFP⁺ T cells and among i. splenocytes. * = p < 0.05, ** = p < 0 .01 by the Mann-Whitney U test. Data are from A2⁺ n = 6, Sw n = 5 and A2⁻ n = 4 recipient mice, respectively.