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. 2020 Feb 7;40(2):BSR20193087. doi: 10.1042/BSR20193087

Figure 2. SPAG5 mRNA and protein expression in ovarian cancer tissues and matched noncancerous tissues.

Figure 2

One-step quantitative real-time polymerase chain reaction (qPCR), Western blotting (WB), and immunohistochemistry (IHC) were performed to evaluate SPAG5 mRNA and protein expression in ovarian cancer tissues and matched noncancerous tissues. SPAG5 mRNA expression in ovarian cancer tissues was significantly higher than that in matched tumor-adjacent noncancerous tissues (P = 0.002) when normalized to the internal control GAPDH (A). SPAG5 protein expression in ovarian cancer tissues was higher than that in matched tumor-adjacent noncancerous tissues by WB (B) and IHC (C). IHC scoring data showed that SPAG5 protein expression in ovarian cancer tissues was significantly higher than that in matched tumor-adjacent noncancerous tissues (P < 0.001).