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. 2020 Feb 7;40(2):BSR20182121. doi: 10.1042/BSR20182121

Figure 2. miR-128 promotes the osteogenic differentiation of rBMSCs in vitro.

Figure 2

(A) The expression levels of miR-128 examined in rBMSCs transfected with miR-128 mimic or mimic-NC, miR-128 inhibitor or inhibitor NC. (B) CCK-8 assay results obtained at 0, 12, 24, 48, and 72 h after miR-128 mimic or inhibitor transfection. (C and D) ALP activity and alizarin red staining performed on day 7 and 28. (E) Osteogenesis enhancement as evaluated by qRT-PCR analysis of osteogenic-related genes (RUNX2, BMP-2, COLIA1, normalized to β-actin) 4 and 14 days after transfection. (F) Western blot analysis of RUNX2, BMP-2, and COLIA1; *P < 0.05, NS, not significant.