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. 2020 Feb 7;40(2):BSR20182121. doi: 10.1042/BSR20182121

Figure 3. MiR-128 targets DKK2 negatively and regulates Wnt signaling pathway.

Figure 3

(A) Schematic diagram illustrating the design of the luciferase reporters with WT 3′-UTR or 3′-UTR-mut for DKK2. (B and C) Association analysis between miR-128 and mRNA expression levels (determined by qRT-PCR) and protein levels (determined by Western blot analysis) of DKK2. (D) Effects of a miR-128 mimic on luciferase activity in rBMSCs transfected with either the WT 3′ UTR reporter or the 3′ UTR-mut reporter for DKK2. (E) mRNA expression levels of Lef-1 and Tcf-4 as analyzed by qRT-PCR, normalized to β-actin. (F) mRNA expression levels of CyclinD1 and Axin2 analyzed by qRT-PCR, normalized to β-actin. (G) Lef-1, Tcf-4, CyclinD1, Axin2 and nucleus β-catenin protein levels were analyzed by Western blot, normalized to β-actin; **P <0.01, *P <0.05, NS, not significant.