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. 2019 Oct 7;38(2):225–232. doi: 10.1038/s41587-019-0266-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Homozygous polled (Polled, 591 bp), homozygous horned (Horned, 389 bp) and negative PCR controls are shown at the right. The offspring of the genome-edited polled bull are heterozygous POLLED and the Horned Hereford controls are homozygous HORNED. This PCR was carried out for the 28 animals sequenced in this study.