Fig. 4. The number of SNP variants relative to the ARS-UCD1.2 bovine reference genome derived from a Hereford cow.

The Hereford cow was L1 Dominette 01449 (ref. ¹³). Males (squares) and females (circles) are shown in four study groups. a, GH.H.: genome-edited polled bull (RCI002, black) was bred to Horned Hereford cows (RC.dams1–6, brown) and produced six polled offspring (RC.calves1–6, blue). b, H.H.: Horned Hereford bulls (HH.sire1 and HH sire23, purple) were bred to Horned Hereford cows (HH.dams1–3, brown) and produced three horned offspring (HH.calves1–3, blue). c, Ho.H.: Horned Holstein sire (HO1, pink) was bred to Horned Hereford cows (HO1.dams1–3, brown) by artificial insemination and produced three horned offspring (HO1.calves1–3, blue). d, Carlson: original sequences of cell lines (CL2122.org (black) and CL2120.org (red)) that were edited to produce bulls, RCI002.org (black) and RCI001.org (red), respectively, as reported by Carlson et al.². RCI002 and RCI002.org are data from the same genome-edited bull sequenced by two different sequencing laboratories in different years.