Fig. 5.

Expression of 5-LOX, ED-1, OX-42, and GFAP in the cerebral cortex after embolic stroke (ES). (a) Representative Western blots of 5-LOX, ED-1, OX-42, and GFAP in the ipsilateral (ipsi) and contralateral (contra) sides for sham and ES rats at 3, 24, and 72 h post-ES. (b) Comparison of cortical expression of 5-LOX, ED-1, OX-42, and GFAP between sham ipsi and ES ipsi, n = 3–5 per group. The ES group at 3 h was used as the reference group which was set to 1. Expressions of 5-LOX, ED-1, and OX-42 were consistently not detected (nd) in Sham controls. Statistical analysis was performed by two-way ANOVA testing time factor and stroke factor. F(1,14) = 13.83, p < 0.005 (5-LOX); F(1,24) = 35.65, p < 0.0001 (ED-1); F(1,18) = 21.64, p < 0.0005 (OX-42); F(1,14) = 14.14, p < 0.005 (GFAP), for stroke factor. *p < 0.05 and **p < 0.01 vs corresponding sham group by Bonferroni correction. No significance obtained for time factor and interaction in all 4 markers. Total number of animals used was 12