Fig. 3.

Heterogenic effects of DHA dietary supplementation over protein oxidative modifications in the proteins of LSC of G93A mice. Oxidative damage as measured by GSA (a) and AASA (b) contents in LSC proteins shows gender- and age-sensitive profiles. In contrast, CML, a marker of mixed lipooxidative and glycoxidative modifications (c), and CEL, a marker of methylglyoxal-derived modifications (d), generally exhibit decreased levels at 90-day and endpoint stages, irrespective of gender. Noteworthy, MDAL, a marker of lipooxidative modifications, shows a significant decrease in males only at earlier stages (e). Bars indicate mean values, whereas error bars represent ± S.E.M. Significant differences between dietary groups (*p < 0.05, **p < 0.01, ***p < 0.001, p < 0.0001) were evaluated by a Bonferroni post hoc analysis after 2-way ANOVA, accounting age and diet