Fig. 5.

Dietary DHA supplementation leads to changes in synaptic components as well as in DNA and protein repair mechanisms in G93 male mice, without changes in astrogliosis or microglial infiltration. (a) Representative Western blot analyses evaluating levels of syntaxin 3 in LSC. In the right panel, the quantitative analyses of Western blot signals, adjusted to tubulin content. (b) Representative confocal images (left) and quantitative analyses (right) of neurofilament (SMI-32) and ubiquitin immunoreactivities in whole LSC sections of 90-day-old G93A male mice. (c) Representative confocal images (left) and quantitative analyses (right) of DNA repair mechanisms (γH2Ax) and DHA-related decreases in DNA oxidative damage (8-oxo-dG) in whole LSC sections of 90-day-old G93A male mice, measured in immunohistochemistry. (d) Representative confocal images showing a lack of DHA-related changes in GFAP immunostaining in whole SC sections of 90-day-old G93A male mice, quantified in right panels. (e) Representative confocal images showing low-n-3 and low-n-3 + DHA-related decreases in the proportion of Iba1 immunoreactive cells in whole SC sections of 90-day-old G93A male mice, quantified in right panels. In (a), bars indicate mean values, whereas error bars represent ± S.E.M (n = 4 experiments). Points in (b), (c), and (d) indicate different sections, belonging to at least 3 different animals for each dietary treatment. Differences between dietary groups were evaluated by Student’s t test analyses (or χ² test for (e)), being *p < 0.05 and **p < 0.01. Scale bars in (b) and (c) are 1000 μm long and in (d) and (e) are 100 μm and 50 μm long, respectively