Fig. 5.

Immunohistochemical analysis of the effects of the three concentrations of oral tonabersat on LD rats. Orally treated rats showed less Connexin43 immunoreactivity in the retina for all three dose levels (b-d) compared to the vehicle group (a). Iba-1 immunolabeled cells showed low activation (sprouting) in the IPL of the retina of tonabersat-treated rats for all three doses (f-h) compared to vehicle-treated rats (e) although a slight increase in Iba-1 reactivity in the lowest 0.26 mg/kg oral dose was evident. GFAP immunoreactivity did not increase in the retina of 0.8 mg/kg tonabersat (k) and 2.4 mg/kg tonabersat (l) as compared to vehicle rats (i). In the lowest 0.26 mg/kg oral dose group, there was slightly increased GFAP labelling but this was still less expression than for the vehicle alone group (j). Abbreviation: GCL, ganglion cell layer; IPL, inner plexiform layer. Scale bar, 50 μm