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. 2020 Jan 21;117(5):2579–2587. doi: 10.1073/pnas.1909761117

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Copyright © 2020 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 7. — (A) In vitro processing assay of pre-miRNA of DICER1, Δhel-DICER1, and ΔPAZ-DICER1 purified from HEK 293T cells. (B) Immunoblotting of HeLa and primary human RPE (hRPE) after transient transfection with plasmids to express GFP (pMaxGFP), Δhel-DICER1 (pΔhel-DICER1), or full-length human DICER1 (pDICER1). (C) Time course of Δhel-DICER1 expression in hRPE cells after transient transfection. Note faint detection of Δhel-DICER1 at 4 and 8 h after transfection. (D) Dose-dependent effect of dsRNA cotransfection on Δhel-DICER1 in hRPE. (E) Expression of endogenous and Δhel-DICER1 in primary hRPE 24 and 48 h after transfection with indicated DICER1 constructs.