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. 2020 Jan 21;117(5):2570–2578. doi: 10.1073/pnas.1915047117

Fig. 5.

Fig. 5.

Early-life antibiotics treatment impacts later-life thymic PLZF+ cell distribution. (A) C57BL/6 breeders were given antibiotics (vancomycin, neomycin, and ampicillin) in drinking water at the time of mating. Pups born of these “altered microbiota” breeders were analyzed at 14 d of age (Inf-Ctrl and Inf-Abx) or weaned at 21 d and housed in conventional isolators without antibiotics and analyzed at age 48–55 d (Ad-Ctrl and Ad-Abx). (B, Left) Representative flow cytometry plots showing PLZF-expressing cells and (Right) frequency (Inf-Ctrl n = 10; Inf-Abx n = 13; Ad-Ctrl n = 9; Ad-Abx n = 6) and total numbers of PLZF+ cells (Inf-Ctrl n = 10; Inf-Abx n = 13; Ad-Ctrl n = 9; Ad-Abx n = 6). Effect size: Inf-Ctrl vs. Inf-Abx: 1.90 and Ad-Ctrl vs. Ad-Abx: 2.82. (C) Pie graphs showing distribution of indicated PLZF-expressing cell subsets. (D) Conventionally housed adult (d48–55) C57BL/6 mice were given antibiotics (vancomycin, neomycin, and ampicillin) in drinking water for 2 wk. (E, Left) Representative flow cytometry plots showing PLZF-expressing cells, (Right) frequency (Ctrl n = 7; Abx n = 6), and total numbers of PLZF+ cells (Ctrl n = 7; Abx n = 6). (F) Pie graphs showing distribution of indicated PLZF-expressing cell subsets in adult mice treated with antibiotics. Data are representative of two independent experiments. Bars are mean ± SEM.