Fig. 5.

Prophylactic efficacy of SGM1.7. (A) Antimetacyclic-specific rSGM1.7 IgG and naive mice IgG coinoculated with 500 SG parasites intraperitoneally in two groups of mice, respectively. Parasitemia was followed via eye bleeds. (B and C) Two independent groups of mice vaccinated with metacyclic-specific rSGM1.7 or epimastigote-specific rSGE1 were challenged with 500 SG parasites injected i.d. in the ear. As a control, nonvaccinated age-matched groups of mice were similarly infected. Parasitemia was followed via eye bleeds. (D) Infections in mice vaccinated with metacyclic-specific rSGM1.7 or age-matched naive controls were initiated through a single infected fly bite intraperitoneally. For statistical analysis, a mixed-effect linear model was fitted, where the logarithm in base 10 of the parasitemia counts was taken as the dependent variable. For day-to-day pairwise comparison of parasitemia between vaccinated and nonvaccinated mice, significance was evaluated with Tukey’s contrasts. Statistically different data points are shown by asterisks: * > 0.05, *** > 0.0001. Complete statistical results are available in Dataset S4.