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. 2020 Jan 22;117(5):2588–2596. doi: 10.1073/pnas.1921404117

Fig. 1.

Fig. 1.

High levels of p38α expression correlate with malignancy and poor prognosis. (A) Boxplots for expression levels of MAPK14 mRNA comparing paired adenocarcinomas and healthy tissues (n ≥ 49 per group). a.u., arbitrary units. (B) Kaplan–Meier plots showing a univariate analysis of overall survival of lung adenocarcinoma patients stratified by high or low MAPK14 mRNA expression (n ≥ 39 per group). (C) Kaplan–Meier plots displaying a recurrence-free survival over time of lung adenocarcinoma patients stratified by high or low MAPK14 mRNA expression (n ≥ 26 per group). (D) Representative images and boxplot of the percentage of phospho-p38+ cells in lung adenocarcinoma tumors (n = 17), paired normal tissue (n = 14), and healthy lung parenchyma (n = 5) from a human tissue array. (Scale bar, 200 μm.) (E) The same samples as in D were stained with a p38α antibody by immunofluorescence. DAPI marks nuclei. (F) Representative image of a murine KrasG12V-driven lung tumor stained with phospho-p38 antibody. (Scale bar, 200 μm.) The histogram shows the percentage of phospho-p38+ cells in tumors and in healthy lung parenchyma (n = 5 healthy tissues and n = 21 tumors from 6 different mice). Data represent average ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.