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. 2020 Jan 22;117(5):2597–2605. doi: 10.1073/pnas.1917042117

Fig. 2.

Fig. 2.

Characterization of AgP47Rec in A. gambiae and effect of dsRNA-mediated silencing on P. falciparum infection. (A) Neighbor-joining phylogenetic tree (without distance correction) of AgP47Rec orthologs and related proteins in other dipterans, including Drosophila melanogaster (Dm), P. papatasi (PhPa), C. marinus (CIMa), A. darlingi (AnDa), A. gambiae (Anga), Aedes albopictus (AeAl); Aedes aegypti (AeAe); and C. quinquefasciatus (CxQu). (B) Expression of AgP47Rec in A. gambiae midguts of sugar fed (SF), control blood fed (C), or P. falciparum-infected blood fed (I) mosquitoes at different times. (C) Western blot of AgP47Rec protein expression in A. gambiae midgut pellet (Upper) and midgut cytosol (midgut supernatant, Lower) from blood-fed mosquitoes 12, 24, 48, 72 h PF, or sugar fed (0 h). (D) qPCR quantitation of AgP47Rec mRNA in whole mosquitoes after injection of AgP47Rec dsRNA (dsP47Rec) or control LacZ dsRNA (dsLacZ). (E) Western blot of AgP47Rec in midguts from mosquitoes after injection of AgP47Rec dsRNA (Rec) or control LacZ dsRNA (LcZ). (F) P. falciparum oocyst infection levels in midguts from mosquitoes injected with AgP47Rec dsRNA (dsP47Rec) or control LacZ dsRNA (dsLacZ). n, number of mosquitoes analyzed; Pev., prevalence of infection. ****P < 0.0001 Mann–Whitney U test, **P < 0.01 χ-squared test.