Fig. 2.

Enhanced beige adipocytes in the adipose tissue of Scd1-deficient mice is not related to white-to-beige transition. (A) The ratio of tissue mass (BAT, iWAT, and gWAT) to body weight of 8-wk-old control (Scd1^flox) and F^CreScd1^flox mice (n = 3 for each group, repeated two times). (B) H&E staining of representative sections of iWAT, gWAT, and BAT from 8-wk-old control (Scd1^flox) and F^CreScd1^flox mice. (Scale bars, 100 µm.) (C) Relative mRNA levels of thermogenesis- and adipogenesis-related genes in iWAT and BAT of control (Scd1^flox) and F^CreScd1^flox mice (n = 4 for each group, repeated two times). (D) Body weight of control (Scd1^flox) and F^CreScd1^flox mice fed an HFD for 12 wk (n ≥ 4 for each group, repeated three times). (E and F) The ratio of fat mass and lean mass to body weight (E) and insulin sensitivity (F) of control (Scd1^flox) and F^CreScd1^flox mice fed an HFD for 12 wk (n = 3 for each group, repeated three times). (G) Daily O₂ consumption, CO₂ production, and heat production of control (Scd1^flox) and F^CreScd1^flox mice fed an HFD (n = 4 for each group, repeated two times). (H) Body weight of control (Scd1^flox) and U^CreScd1^flox mice fed an HFD for 8 wk (n ≥ 4 for each group, repeated three times). (I) Heat generation of the light cycle and the dark cycle in control (Scd1^flox) and U^CreScd1^flox mice fed an HFD (n = 4 for each group, repeated two times). (J) H&E staining of representative sections of iWAT, gWAT, and BAT of control (Scd1^flox) and U^CreScd1^flox mice fed an HFD for 8 wk. (Scale bars, 100 µm.) Data are shown as mean ± SEM. *P < 0.05 and ***P < 0.001.