Fig. 1.

Schematic representation of cellulosomal proteins. (A) Biomimetic scaffoldins (CipA) with varying numbers of cohesins and CBMs. In a scaffoldin name, “B” represents the number of CBMs and “C” represents the number of cohesins. For example, CipA1B9C means one CBM and nine cohesins are present in a CipA. (B) Fungal cellulases fused with dockerin T. (C) Outer layer scaffoldin protein B (OlpB) with seven type II cohesins and the ScGPI cell surface anchor. (D) ScafT containing a CBM and a cohesin T. (E) Overview of the engineered yeast hosts used in this study. (F) Schematic diagram of the engineered cellulosome complex with 63 enzymes on the cell surface of K. marxianus.