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. 2020 Feb 8;15:14. doi: 10.1186/s13000-020-00929-9

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© The Author(s) 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — a Top panel: Western blotting and RTq-PCR analysis of Foxm1 expression in Eca109 and Ec9706 cells, respectively. Bottom panel: Quantitative analysis *P<0.05; b Top panel: Foxm1 expression of three groups of EC cells following FoxM1 silencing. Bottom panel: Quantitative data for the top panel. *P<0.05; c Top panel: Western blotting to detect Foxm1 and β-catenin expression in the nuclei of the three groups EC cells after FoxM1 silencing. Bottom panel: Quantitative data for the top panel. *P<0.05; d Top panel: Western blotting to detect the expression of Foxm1, β-catenin, tcf4, and E-cad in the three groups of EC cells after FoxM1 silencing. Bottom panel: Quantitative data of the top panel. *P<0.05; e Top panel: RT-PCR to measure the mRNA levels of Foxm1, β-catenin, tcf4 and E-cad in the three groups of EC cells after FoxM1 silencing. Bottom panel: quantitative data for the top panel. *P<0.05