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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 Oct 31;40(1):128–144. doi: 10.1161/ATVBAHA.119.313169

Figure 6. Increased plasma protein leakage in the cortex of apoE4-TR mice.

Figure 6.

A-E, The levels of collagen IV (A; apoE, p<0.0001; sex, p=0.4401, apoE × sex, p=0.7307), claudin-5 (B; apoE, p=0.4365; sex, p=0.5057, apoE × sex, p=0.4332), occludin (C, apoE, p=0.7529; sex, p=0.8528, apoE × sex, p=0.9210), fibrinogen (D, apoE, p=0.0002; sex, p=0.9759, apoE × sex, p=0.5057) and IgG (E, apoE, p=0.0428; sex, p=0.5767, apoE × sex, p=0.6582) were determined by ELISA in apoE3-TR (male; N=8, female; N=9) and apoE4-TR mice (male; N=8, female; N=8) at 22 months of age. The measurement was normalized by protein concentration in each of the samples. Data in A-E are presented as mean ± s.e.m. Each dot represents a measurement from one mouse. *P<0.05, **P<0.01 by Tukey-Kramer’s post-hoc analysis of two-way ANOVA. N.S., not significant among groups. F-G, The correlations between the levels of leaked plasma protein (fibrinogen and IgG) and that of collagen IV calculated across the male or female apoE3-TR (red circle) and apoE4-TR (blue circle) mice were assessed through nonparametric Spearman correlation analysis. The correlation coefficient (R2) and p value are shown in each panel.