2. Pcyt2 knockdown redirects DAG from PE to TG synthesis.

The suppression of Pcyt2 activity results in decreased flux through the CDP-ethanolamine pathway and thus, CDP-ethanolamine production is reduced. Inadequate CDP-ethanolamine pools limit the ability of EPT to use of DAG as a substrate in the final reaction of PE production and causes DAG to accumulate in the cell. Consequently, DAG is shifted from a substrate for PE to TG synthesis, leading to increased production and accumulation of TG. EK: ethanolamine kinase; P-ethanolamine: phosphoethanolamine; ECT/Pcyt2: CTP:phosphoethanolamine cytidylyltransferase; EPT: CDP-ethanolamine:1,2-diacylglycerol ethanolaminephosphotransferase; DAG: diacylglycerol; TG: triglyceride.