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High-throughput single-molecule assay for host-guest chemistry. (A) Experimental setup. Barrels depict host molecules. (B) Structures of host (i & ii) and guest (iii-vi) molecules used in the mechanical unfolding experiments. CB7 stands for cucurbit[7]uril, βCD stands for β-cyclodextrin, and Ad stands for adamantane (blue). Hexyl group is shown in green.
