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. Author manuscript; available in PMC: 2020 May 25.
Published in final edited form as: Org Lett. 2019 Aug 9;21(17):6790–6794. doi: 10.1021/acs.orglett.9b02406

Figure 2.

Figure 2.

Representative SDS–PAGE and MS analysis of tri-Ub ABPs. (A) Coomassie-stained SDS–PAGE gel showing the formation of probe 11a using Ub-BVK and di-Ub 10a. ESI-MS analysis of purified 11a (observed deconvoluted m/z ratio for [M + H]+ is 25845.8; calculated m/z is 25846.6). The di-Ub species present along with Ub-BVK 9 is formed by self-condensation of Ub-MESNa 7 while concentrating the sample. (B) SDS–PAGE gel showing the formation of 11b starting from Ub-BVK and di-Ub 10b. ESI-MS analysis of purified 11b (observed deconvoluted m/z ratio for [M + H]+ is 25817.7; calculated m/z is 25817.1).