Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 30;98(2):309–320. doi: 10.1007/s00109-019-01865-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Dicer-deficient CD8⁺ thymocytes overexpress IFN-γ. (a) Representative plot (a) and quantification (b) of flow cytometry analysis of IFN-γ and IL-17 protein levels in thymic CD8⁺ T cells of dicer^lox/lox and lckCre dicer^Δ/Δ mice (n = 4) upon ex vivo stimulation with PMA and ionomycin for 4 h at 37 °C with the addition of Brefeldin A. (c) RT-qPCR analysis of Ifng, Tbx21 and Eomes in thymic CD8⁺ T cells of dicer^lox/lox relative to lckCre dicer^Δ/Δ mice, normalized to housekeeping Actb mRNA. * P ≤ 0.05