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. Author manuscript; available in PMC: 2020 Oct 4.
Published in final edited form as: Sci Immunol. 2019 Oct 4;4(40):eaax1215. doi: 10.1126/sciimmunol.aax1215

Figure 3. Cell-intrinsic BMAL1 is required for ILC3 homeostasis in the intestine.

Figure 3.

A. Representative histogram overlay of BMAL1 expression in SI-LP ILC3 and Th17 cells from BMAL1fl/fl mice and BMAL1ΔRorc mice. ILC3 were gated as live CD45+Lin−CD90.2+CD127+GATA-3−RORγt+, Th17 cells were gated as live CD45+CD3ε+CD4+RORγt+IL-17A+. B. Bar graphs of MFI of RORγt and NFIL3 in SI-LP ILC3 from BMAL1fl/fl mice and BMAL1ΔRorc mice (representative of 3 independent assays). C. Representative flow cytometry plots and bar graphs of frequency and cell numbers of SI-LP ILC3 from BMAL1fl/fl mice and BMAL1ΔRorc mice. Cells were gated on live CD45+Lin−CD90.2+CD127+ (representative of 4 independent assays). D. Representative flow cytometry plots and bar graphs of frequency and cell numbers of T-bet+ ILC3 from SI-LP of BMAL1fl/fl mice and BMAL1ΔRorc mice. Cells were gated on live CD45+Lin−CD90.2+CD127+GATA-3−RORγt+ (representative of 4 independent assays). Results are shown as the mean ± s.e.m. Statistics are calculated by unpaired two-tailed Student’s t-test.