Figure 9.

Anti‐ricin MAbs function via the mannan‐dependent uptake pathway in LSECs. Freshly isolated LSECs were seeded in 96‐well microtiter plates and pulsed for 2 h with ricin plus indicated anti‐RTA (panel A) or anti‐RTB (panel B) MAbs, in the presence of α‐mannan (1 mg/ml) or lactose (0.1 M). Cell viability was normalized to LSECs that had not been treated with ricin toxin. In all cases, the addition of lactose resulted in a significant increase in cell viability, as compared with the control (ricin only). Mannan addition impacted cell viability in only 1 instance (MH3). Kruskal–Wallis with Tukey's multiple comparisons test (GraphPad 7) was applied to account for non‐normal distribution of data