Fig. 3.

EV-miR-7 targets neuronal NLGN2. a Putative miR-7 binding site in NLGN2 3’UTR. b HEK293 cells were transfected with 200 nM mature miR-7 oligos using Lipofectamine 2000 for 24 h. Transfection efficiency was determined using qPCR and was normalized to U6 small nuclear RNA (U6). c Cell viability was measured using MTT assay. d Relative luciferase activity of WT/3′UTR mutant constructs of NLGN2 co-transfected with miR-control/miR-7. d, e Western blot analysis of NLGN2 protein levels in rat primary neurons treated with EVs (100 EVs per cell) isolated from astrocytes loaded with control or miR-7 oligo (2.5 pmol per well) (e) and EVs isolated from astrocytes under indicated conditions (f). *p < 0.05 vs control