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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Res. 2019 Dec 17;1867(4):118625. doi: 10.1016/j.bbamcr.2019.118625

Figure 12.

Figure 12.

Effect of p44/42 or Akt inhibition on Bn-stimulated cell-growth in NSCLC cells. (A, B) H441 and Calu-3 cells were incubated with/without Bn (1 μM), in the presence or absence of PD98059 (10 μM), U0126 (10 μM), Wortmannin (1 μM) or LY294002 (10 μM) for 24 hr. Cell-proliferation was assessed by CCK-8 assay (A), and the results were confirmed by MTS assay (B). Data was presented as % increase with Bn stimulation against basal proliferation. Basal stimulation for the two cell-lines were 100% for control, 72%-83% for PD98059, 65%-80% for U0126, 68%-80% for Wortmannin, and 56%-73% for LY294002, respectively, and maximal stimulation with Bn for the two cell-lines were 116%-121% for control, 72%-86% of control with PD98059, 69%-85% for U0126, 83%-94% for Wortmannin, and 69%-87% for LY294002, respectively. The mean value ± SEM of 4 experiments is indicated. * P < 0.05 vs. no inhibitor (E, F). These results are representative of 2 others.