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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Kidney Int. 2019 Mar 29;96(3):597–611. doi: 10.1016/j.kint.2019.03.014

Figure 5|. A subset of tdTomato+ parietal epithelial cells (PECs) that have migrated to the tuft after podocyte loss acquire a podocyte fate and begin to express the podocyte protein podocin.

Figure 5|

Representative confocal micrographic images of enhanced green fluorescent protein (EGFP)+ (green), tdTomato (red), and podocin (blue) at baseline, day 28 (D28), and day 56 (D56) of focal segmental glomerulosclerosis (FSGS). Panels labeled with numbers 1 and 4 represent EGFP (green); 2 and 5, tdTomato (red); and 3 and 6, immunofluorescence co-stained with the podocyte marker podocin (blue). (a) At baseline, the majority of EGFP+ cells (a1, a4) co-localized with podocin (a3, a6) (marked with dashed arrows), and tdTomato+ PECs are detected along Bowman’s capsule (a2, a5) (marked with white arrowheads). (b) At day 28 of FSGS, a tdTomato+ PEC (b2, b5) (marked with solid arrows in dashed box) has migrated onto the glomerular tuft, has transdifferentiated to a podocyte fate, and co-expresses EGFP (green) (b1, b4) and podocin (blue) (b3, b6), creating a pink/yellow color (b). (c) At FSGS day 56, a tdTomato+ PEC (c2, c5) has migrated onto the glomerular tuft, has transdifferentiated to a podocyte fate, and co-expresses EGFP (green) (c1, c4) and podocin (blue) (c3, c6), creating a pink/yellow color (c, marked with solid arrows). Bars = 25 μm or 5 μm (insets).