Fig. 4.

In the absence of lnt, the integrity of the outer membrane (OM) is compromised. Overnight cultures of wild-type and lnt mutant for A. baylyi (a) and A. baumannii (b) strains were spread onto the surface of an LB agar plate. After allowing the cultures to dry, filter discs impregnated with the indicated antibiotics [bacitracin (10 units), novobiocin (30 µg), vancomycin (30 µg) and polymyxin B (300 units)] were placed on the agar surface. Zones of growth inhibition (in mm) were measured after overnight growth at 30 °C. Bar charts represent the mean of triplicate measurements, with error bars indicating standard deviation from the mean. *, P≤0.05 by Student’s t-test.