Figure 4.

Plasma and skeletal muscle APA activity. Enzyme activity of aminopeptidase A (APA) measured in the membrane fraction isolated from the skeletal muscle and the blood plasma control obese Zucker rats and in obese Zucker rats receiving AVE0991 via osmotic minipumps for two weeks. Gene expression of aminopeptidase A (APA) in musculus quadriceps determined by real-time PCR. Data were normalized to the gene expression of 40S ribosomal protein S29 (Rps29) whose expression was not altered by the treatment. Data presented as mean ± S.E.M. were analysed by Student's t-test, ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001.