Figure 5.

Renin receptor in the skeletal muscle of Zucker rats. Gene expression of the renin receptor (ReR) at the level of mRNA (a) and protein with representative Western blots (b); mRNA expression of the transcription factor promyelocytic leukemia zinc finger (PLZF, Zbtb16) and furin in musculus quadriceps of control obese Zucker rats and in obese Zucker rats receiving AVE0991 via osmotic minipumps for two weeks. Levels of mRNA were determined by real-time PCR. Data were normalized to the gene expression of 40S ribosomal protein S29 (Rps29) whose expression was not altered by the treatment. The amount of renin receptor protein was quantified by Western blot method. The signal intensities of the proteins of interest were normalized to the sample's total protein content stained with Coomassie Brilliant Blue. Data are presented as mean ± S.E.M. Results were analysed by Student's t-test, ^∗p < 0.05.