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. Author manuscript; available in PMC: 2020 Feb 10.
Published in final edited form as: J Am Chem Soc. 2020 Jan 16;142(4):1911–1924. doi: 10.1021/jacs.9b11093

Figure 3.

Figure 3.

Analysis of the zero-field and 53 mT Mössbauer spectra of reconstituted Bt MiaB after incubation with tRNA and SAM for 20 min. (A) [0–53 mT] difference spectrum (vertical bars), and simulation of the MiaB [3Fe-4S]0-like cluster in 53 mT using the parameters listed in Table 2 and scaled to −27% of total 57Fe absorption (black line). Arrows indicate the onset of the broad absorption features arising from the magnetic hyperfine splitting of the MiaB [3Fe-4S]0-like cluster. (B) Zero-field reference spectrum of the MiaB [3Fe-4S]0-like cluster (vertical bars). Black line is the simulation of the MiaB [3Fe-4S]0-like cluster in zero field with parameters from Table 1, solution 1 (30% of total intensity). Individual contributions from the three sites are shown above the zero-field reference spectrum (site 1 (green), site 2 (red), site 3 (blue); 10% of total intensity each).