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. 2020 Jan 2;295(6):1426–1438. doi: 10.1074/jbc.RA119.011638

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© 2020 Burke et al.

Author's Choice—Final version open access under the terms of the Creative Commons CC-BY license.

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Figure 1. — RNase L inhibits SG but not PB assembly and promotes RLB assembly. A, Western blotting analysis of PKR and RNase L in parental (WT), RNase L–KO (RL-KO), PKR-KO, and RNase L/PKR double knockout (RL/PKR-KO) A549 cells. B, IF for G3BP1 (SG marker) and DCP1b (PB marker) in the indicated A549 cell lines transfected with or without poly(I·C). To calculate the percentage of cells with G3BP1+ foci (RLBs or SGs), 25–195 cells from between 3 and 15 fields of view were analyzed. C, histogram of the number of G3BP1-positive foci binned by area as represented in B. D, histogram of the number of DCP1b-positive foci binned by area as represented in B. E, similar to B but depicting RLB and PB association. F, IF for G3BP1 and FISH for poly(A)+ RNA in WT cells 4 h post-poly(I·C).