Figure 7.

Loss of YAP1 affects oxidant-mediated induction of MMT1 but not MMT2 expression. A, WT cell transformed with MMT1-lacZ or MMT2-lacZ were grown in the presence or absence of 0.005% H₂O₂ for 2 h, and β-gal activity was measured. Error bars represent S.D. n = 4. B, WT, Δyap1, Δyap3, Δyap5, and Δyap7 cells transformed with MMT1-lacZ were grown with 0.005% H₂O₂ for 2 h, and β-gal activity was measured. Error bars represent S.D. n = 6. C, cells expressing truncation mutants as in Fig. 4 were grown in the presence or absence of H₂O₂, and β-gal activity was measured. Error bars represent S.D. n = 4. D, WT, Δyap1, Δaft1, and Δyap1Δaft1 cells serially diluted onto CM plates in the presence or absence of 0.0006% or 0. 00125% H₂O₂ were grown for 2 days, and images were captured. ***, p ≤ 0.001; ****, p ≤ 0.0001.