Figure 7.

THP-1 human macrophages were differentiated with PMA for 72 h followed by a 24-h rest period in PMA-free medium. The cells were stimulated with LPS (10 ng/ml) immediately followed by 10 μm HS-243. 105 cytokines/chemokines were profiled from the supernatant of the media. a and b, significant changes seen in MIP-3a, Tim-3, uPAR, RANTES, Osteopontin, MMP-9, MIP-1a, MCP-1, I-TAC, IL-8, GDF-15, IP-10, and chitinase-3–like 1 protein expression (n = 3–4). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.