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Kinetics parameters for recombinant NnOMT1 (6OMT) and NnOMT5 (6OMT and 7OMT) determined at a fixed saturating concentration of SAM
Values represent the mean ± S.D. of three independent measurements.
| Enzyme | Substrate | Km | Vmax | kcat | kcat/Km |
|---|---|---|---|---|---|
| μm | nmol min−1 mg−1 protein | s−1 | m−1 s−1 | ||
| NnOMT1 | (R,S)-Norcoclaurine | 20 ± 2 | 52 ± 2 | 0.0336 | 1686 |
| NnOMT5 | (R,S)-Norcoclaurine | 152 ± 36 | 9 ± 1 | 0.0058 | 38 |
| (S)-N-Methylcoclaurine | 13 ± 3 | 30 ± 2 | 0.0191 | 1465 |
