Figure 2.

Knockdown of TgUroD causes a defect in parasite proliferation. A, Western blotting of the rTgUroD-c-Myc line grown in the presence of ATc for 0–3 days and probed with antibodies against c-Myc (to detect rTgUroD-c-Myc) and TgGRA8 (as a loading control). B–D, fluorescence growth assays depicting parasite proliferation in (B) parental Tomato/TATiΔku80, (C) tomato/rTgUroD, and (D) cTgUroD-HA/Tomato/rTgUroD strain parasites grown in the absence (blue) or presence (orange) of ATc. Proliferation is expressed as a percentage of that measured in parasites grown in the absence of ATc on the final day of each experiment. Data depict the mean ± S.D. from three technical replicates and are representative of three independent experiments.